innoVation Facts – Cardiac Maturation Cues (Part 3)

The last part of our innoVation Facts series dealing with cardiac maturation cues focused on gene expression analysis of human iPSC-derived cardiomyocytes (hiPSC-CMs) cultured either on physiological FLEXcyte or regular cell culture plates.

The data shows increased expression levels of ACTC1 and ACTN2 genes (both producing cytoskeleton-related proteins) as well as the MYH7 gene (important for cardiac contraction) when hiPSC-CMs are plated on physiological FLEXcyte plates that resemble in vivo conditions of the human heart.

These changes in expression of genes relevant for contraction and cytoskeleton substantiate the phenotypic and functional changes of hiPSC-CMs shown in part 1 and 2 where mature actin structures and an increased contraction amplitude was shown.

Hence, the combination of FLEXcyte 96 plates and hiPSC-CMs give rise to a mature functional and phenotypic state that unveils the full potential of hiPSC-CMs as human-based tool for reliable non-clinical cardiac risk evalution.