FLEXcyte 96 – Call for Proposals
Analyze the contractility of your human iPSC-derived cardiomyocytes or other contractile cells in vitro: The FLEXcyte 96 technology utilizes a disposable unique 96-well plate with incorporated 10 µm polydimethylsiloxane (PDMS) membranes (FLX-96 plates): The cells adhere as monolayers on the flexible substrate. This unique technology allows for recordings in a native-like environment.
Principle: The contractility is measured via movement of the utilized PDMS membranes: While being deflected by the weight of the culture medium, rhythmic contraction of the contractile cells (e.g. cardiomyocytes) lift the membranes in the 96-well upwards. By measuring the changes in deflection, the mechanical stress can be calculated.
Nanion Technologies GmbH will provide a complete instrument for a 6-month project and an intense training on the method and innoVitro GmbH will provide a bundle of consumables. Furthermore, Nanion and innoVitro scientists will support the project intensely.
Projects considered for sponsorship are the development of a new FLEXcyte-based assays or a scientific question concerning structural and functional contractility recordings in cardiotoxicity, safety and efficacy screening and basic research.