Quantitative Live-Cell Analytics
Cellular reactions beyond biochemical endpoints play a pivotal role for lead optimization, efficacy, safety and toxicity testing, regardless of the therapeutic area your project is focussed on.
With the AtlaZ system (Nanion Technologies), we can accelerate your research on cellular level by analyzing compound-induced effects of small molecules, biopharmaceuticals, vector-based or immune therapeutics based on impedance technology. A large variety of cellular features can be monitored for several weeks, including cell adhesion and proliferation, (CAR-T related) cytolysis, G-protein coupled receptor signaling, morphology, and barrier function (TEER).
To obtain a comprehensive understanding of your drug effect, investigation of cellular activity is performed via real-time monitoring in a label-free manner, providing access to kinetic and phenotypic information without the need for external dyes or markers. Simultaneous or independent recording of data from up to six 96-well plates (n=576) allows us to perform high throughput investigations cost and time-effectively.
The AtlaZ methodology relies on electrical impedance spectroscopy. Unique 96-well cell culture plates with integrated gold electrodes allow for quantitative live-cell analysis by measuring the impedance (Ohm) of adherent cells. The cell signal value offers information on cell adherence, proliferation or cell death.
A frequency spectrum ranging from 0.1 kHz up to 100 kHz enables the analysis of a unique richness of cellular information, such as barrier integrity, cytolysis, cell monitoring or Transepithelial Electrical Resistance (TEER). A simultaneous assessment of all frequencies is feasible to speed up live cell analytics.
