July’s innovation Series article by Luo and Li et al., 2020 focuses on the role of type 2 ryanodine receptor (RYR2), an essential calcium channel required for excitation-contraction coupling in cardiomyocytes during human embryonic cardiac development.
Read the article to find out how human iPSC-cardiomyocyte RYR2 -/- lines were generated via CRISP/Cas9 and compared to control lines. The findings show that RYR2 is not required for cardiomyocyte lineage commitment but is important for cardiomyocyte survival and contractile function. The data also demonstrate that the RYR2-/- lines possess an IP3R-mediated alternative calcium regulatory mechanism that compensates for the missing RYR2.
Find the article here